Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

THEMIS Antibody #4482

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 74 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

THEMIS Antibody recognizes endogenous levels of total THEMIS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human THEMIS protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and human primary T cells using THEMIS Antibody.

Background

THEMIS is a recently identified protein found to be critical for T cell development (1-5). It contains two amino-terminal globular domains, a nuclear localization signal, and a carboxy-terminal proline-rich sequence with homology to SH3 binding domains (2,3). THEMIS is detected in both the cytoplasm and the nucleus (2). It is expressed at low levels in mature T cells and during thymocyte development, with expression peaking in CD4+CD8+ double positive cells (1-5). THEMIS is tyrosine phosphorylated downstream of TCR signaling and associates with the adaptor GRB2 and possibly other SH3 domain-containing proximal TCR signaling molecules (1-5). Mice lacking THEMIS have a defect in positive selection that results in decreased numbers of both single positive thymocytes and mature T cells (1-5).

  1. Johnson, A.L. et al. (2009) Nat Immunol 10, 831-9.
  2. Lesourne, R. et al. (2009) Nat Immunol 10, 840-7.
  3. Fu, G. et al. (2009) Nat Immunol 10, 848-56.
  4. Kakugawa, K. et al. (2009) Mol Cell Biol 29, 5128-35.
  5. Patrick, M.S. et al. (2009) Proc Natl Acad Sci U S A 106, 16345-50.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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