Product Pathways - Phosphatases
PTPmu (BK2) Mouse mAb #4485
|W IP||H R Mi (M)||Endogenous||100, 110, 210||Mouse IgG2a|
Reactivity Key: H=Human M=Mouse R=Rat Mi=Mink
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
PTPmu Mouse mAb detects endogenous levels of total PTPmu protein. This antibody does not cross react with other receptor tyrosine phosphatases.
Source / Purification
Monoclonal antibody (isotype: IgG2a) is produced by immunizing mice with a synthetic peptide corresponding to the amino-terminal residues of human PTPmu.
Receptor tyrosine phosphatase PTPmu has an extracellular segment characteristic of adhesion molecules: an MEM domain, an Ig domain and four fibronectin III like (FN III) repeats (1,2). PTPmu is proteolytically cleaved into two noncovalently associated fragments: one is the extracellular domain, the other includes the transmembrane and the intracellular catalytic domains. Both fragments are approximately 100 kDa (3). The extracellular domain mediates cell-cell adhesion in a homophilic, Ca2+ independent manner (1,2). PTPmu associates with multiple cadherins (4). It is able to restore E-cadherin-dependent adhesion in human prostate cancer, and is required for N-cadherin-mediated neurite outgrowth (5,6). The phosphatase activity seems to be essential for the latter function but is dispensable for the former (5,6). PTPmu also associates with and recruits a scaffold protein, RACK (receptor for activated protein C kinase), to cell-cell contact sites (7). Both PKCdelta and src seem to be involved in this process (6,7).
- Gebbink, M. F. et al. (1993) J. Biol. Chem. 268, 16101-16104.
- Brady-Kalnay, S.M. and Tonks, N.K. (1994) J. Biol. Chem. 269, 28472-28477.
- Brady-Kalnay, S. M. et al. (1998) J. Cell Biol. 141, 287-296.
- Hellberg, C. B. et al. (2002) J. Biol. Chem. 277, 11165-11173.
- Burden-Gulley, S.M. and Brady-Kalnay, S.M. (1999) J. Cell Biol. 144, 1323-1336.
- Mourton, T. et al. (2001) J. Biol. Chem. 276, 14896-14901.
- Brandy-Kalnay, S. M. et al. (1995) Receptor protein tyrosine phosphatase PTPmu associates with cadherins and catenins in vivo. J. Cell Biol. 130, 977-986. Applications: IP Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9561 Phospho-β-Catenin (Ser33/37/Thr41) Antibody
- 9562 β-Catenin Antibody
- 9564 Phospho-β-Catenin (Ser45) Antibody
- 9565 Phospho-β-Catenin (Thr41/Ser45) Antibody
- 9371 Phospho-PKC (pan) (βII Ser660) Antibody
- 9921 Phospho-PKC Antibody Sampler Kit
- 9374 Phospho-PKCδ (Thr505) Antibody
- 9375 Phospho-PKCα/β II (Thr638/641) Antibody
- 9377 Phospho-PKCθ (Thr538) Antibody
- 9378 Phospho-PKCζ/λ (Thr410/403) Antibody
- 2055 Phospho-PKCdelta (Tyr311) Antibody
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
For Research Use Only. Not For Use In Diagnostic Procedures.