Cell Signaling Technology

Product Pathways - Protein Stability

CYLD Antibody #4495

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk Endogenous 108 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CYLD Antibody detects endogenous levels of total CYLD protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His334 of human CYLD protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CYLD Antibody.

Background

CYLD is a cytoplasmic deubiquitinating enzyme encoded by a tumor suppressor gene altered in individuals diagnosed with cylindromatosis, a genetic condition characterized by benign tumors of skin appendages (1,2). Functional CYLD deubiquitinase regulates inflammation and cell proliferation by down regulating NF-κB signaling through removal of ubiquitin chains from several NF-κB pathway proteins (3,4). CYLD is a negative regulator of proximal events in Wnt/β-catenin signaling and is a critical regulator of natural killer T cell development (5,6). The transcription factor Snail can inhibit CYLD expression, resulting in melanoma cell proliferation (7).

  1. Biggs, P.J. et al. (1995) Nat Genet 11, 441-3.
  2. Sun, S.C. (2010) Cell Death Differ 17, 25-34.
  3. Regamey, A. et al. (2003) J Exp Med 198, 1959-64.
  4. Glittenberg, M. and Ligoxygakis, P. (2007) Fly (Austin) 1, 330-2.
  5. Tauriello, D.V. et al. (2010) Mol Cell 37, 607-19.
  6. Lee, A.J. et al. (2010) EMBO J 29, 1600-12.
  7. Massoumi, R. et al. (2009) J Exp Med 206, 221-32.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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