Cell Signaling Technology

Product Pathways - Protein Stability

Phospho-CYLD (Ser418) Antibody #4500

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 108 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-CYLD (Ser418) Antibody detects endogenous levels of CYLD protein only when phosphorylated on Ser418.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser418 of human CYLD protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or treated with hTNF-α #8902 (20 ng/ml for 20 minutes), using Phospho-CYLD (Ser418) Antibody (upper) or CYLD Antibody #4495 (lower).

Background

CYLD is a cytoplasmic deubiquitinating enzyme encoded by a tumor suppressor gene altered in individuals diagnosed with cylindromatosis, a genetic condition characterized by benign tumors of skin appendages (1,2). Functional CYLD deubiquitinase regulates inflammation and cell proliferation by down regulating NF-κB signaling through removal of ubiquitin chains from several NF-κB pathway proteins (3,4). CYLD is a negative regulator of proximal events in Wnt/β-catenin signaling and is a critical regulator of natural killer T cell development (5,6). The transcription factor Snail can inhibit CYLD expression, resulting in melanoma cell proliferation (7).

CYLD can be phosphorylated at Ser418 by the noncanonical IKK family member IKKε. Phosphorylation at Ser418 decreases CYLD deubiquitinase activity and is important for IKKε-driven transformation (8).

  1. Biggs, P.J. et al. (1995) Nat Genet 11, 441-3.
  2. Sun, S.C. (2010) Cell Death Differ 17, 25-34.
  3. Regamey, A. et al. (2003) J Exp Med 198, 1959-64.
  4. Glittenberg, M. and Ligoxygakis, P. (2007) Fly (Austin) 1, 330-2.
  5. Tauriello, D.V. et al. (2010) Mol Cell 37, 607-19.
  6. Lee, A.J. et al. (2010) EMBO J 29, 1600-12.
  7. Massoumi, R. et al. (2009) J Exp Med 206, 221-32.
  8. Hutti, J.E. et al. (2009) Mol Cell 34, 461-72.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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