Product Pathways - Glucose Metabolism
IRS-2 Antibody #4502
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 185 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
IRS-2 Antibody detects endogenous levels of total IRS-2 protein. It does not cross-react with IRS-1.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human IRS-2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from CHO IR/4PS cells (transfected with insulin receptor and IRS-2) or CHO IR/IRS-1 cells (transfected with insulin receptor and IRS-1), showing no change in total protein levels with insulin stimulation (100 nM for 5 min), using IRS-2 Antibody (top) or IRS-1 Antibody #2382 (bottom).
Background
Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).
- Sun, X. J. et al. (1991) Nature 352, 73-77.
- White, M.F. (2002) Am. J. Phys. Endocrinol. Metab. 283, E413-E422.
- Withers, D. J. et al. (1998) Nature 391, 900-904.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2382 IRS-1 Antibody
- 2381 Phospho-IRS-1 (Ser307) Antibody
- 2386 Phospho-IRS-1 (Ser612) Antibody
- 2388 Phospho-IRS-1 (Ser636/639) Antibody
- 2389 Phospho-IRS-1 (Ser789) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2384 Phospho-IRS-1 (Ser302) Antibody
- 2390 IRS-1 (59G8) Rabbit mAb
- 3193 Phospho-IRS-1 (Ser612) (L7B8) Mouse mAb
- 3194 IRS-1 (L3D12) Mouse mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
