Product Pathways - MAPK Signaling
TAK1 Antibody #4505
|W F||H M R Mk B (C) (X)||Endogenous||78 to 82||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken X=Xenopus B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
TAK1 Antibody detects endogenous levels of total TAK1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human TAK1. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from untreated or IL-1 treated 293-IL-1R cells, and extracts from HeLa, NIH/3T3, C6, and COS cells, using TAK1 Antibody.
TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2).
- Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
- Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
- Shibuya, H. et al. (1996) Science 272, 1179-1182.
- Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
- Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.
- Morales-Alamo, D. et al. (2012) J Appl Physiol 113, 917-28. Applications: Western Blotting
- Sarkar, S. et al. (2011) Mol Cell Biol 31, 602-15. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.