Product Pathways - MAPK Signaling
TAK1 Antibody #4505
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W F | H M R Mk B (C) (X) | Endogenous | 80 to 82 | Rabbit |
Applications Key:
W=Western Blotting
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
C=Chicken
B=Bovine
X=Xenopus
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
TAK1 Antibody detects endogenous levels of total TAK1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy-terminus of human TAK1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from untreated or IL-1 treated 293-IL-1R cells, and extracts from HeLa, NIH/3T3, C6, and COS cells, using TAK1 Antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells, using Tak1 Antibody (blue) compared to a nonspecific negative control antibody (red).
Background
TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGFbeta, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-kappaB pathway by interacting with TRAF6 and phosphorylating the NF-kappaB inducing kinase (NIK) (2).
- Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
- Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
- Shibuya, H. et al. (1996) Science 272, 1179-1182.
- Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
- Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.
Application References
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Companion Products
- 4531 Phospho-TAK1 (Thr184/187) Antibody
- 4536 Phospho-TAK1 (Thr187) Antibody
- 4537 Phospho-TAK1 (Thr184) Antibody
- 4508 Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
