Cell Signaling Technology

Product Pathways - MAPK Signaling

TAK1 Antibody #4505

Applications Reactivity Sensitivity MW (kDa) Source
W F H M R Mk B (C) (X) Endogenous 78 to 82 Rabbit

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  X=Xenopus  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TAK1 Antibody detects endogenous levels of total TAK1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human TAK1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or IL-1 treated 293-IL-1R cells, and extracts from HeLa, NIH/3T3, C6, and COS cells, using TAK1 Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, using Tak1 Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2).

  1. Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
  2. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
  3. Shibuya, H. et al. (1996) Science 272, 1179-1182.
  4. Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
  5. Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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