Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb #4508

Applications Reactivity Sensitivity MW (kDa) Isotype
W H (M) (R) (C) (X) (Z) (B) Endogenous 82 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  C=Chicken  X=Xenopus  Z=Zebrafish  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb detects endogenous levels of TAK1 only when phosphorylated at threonine 184 and threonine 187. In some cell lysates, the antibody may cross-react with a 40 kDa band of unknown origin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a phosphopeptide corresponding to residues surrounding Thr184 and Thr187 of human TAK1.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 IL-1R cells, untreated or treated for 10 minutes with IL-1, using Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb (upper) or TAK1 Antibody #4505 (lower).

Background

TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2).

  1. Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
  2. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
  3. Shibuya, H. et al. (1996) Science 272, 1179-1182.
  4. Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
  5. Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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