Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb #4508

Applications Reactivity Sensitivity MW (kDa) Isotype
W H (M) (R) (C) (B) (X) (Z) Endogenous 82 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  C=Chicken  B=Bovine  X=Xenopus  Z=Zebrafish
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb detects endogenous levels of TAK1 only when phosphorylated at threonine 184 and threonine 187. In some cell lysates, the antibody may cross-react with a 40 kDa band of unknown origin.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with a phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr184 and Thr187 of human TAK1.

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or IL-1 treated 293 IL-1R cells, using Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb (upper) or TAK1 Antibody #4505 (lower).

Background

TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGFbeta, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-kappaB pathway by interacting with TRAF6 and phosphorylating the NF-kappaB inducing kinase (NIK) (2).

  1. Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
  2. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
  3. Shibuya, H. et al. (1996) Science 272, 1179-1182.
  4. Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
  5. Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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