Product Pathways - MAPK Signaling
Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb #4514
|4514S||100 µl (10 western blots)||---||In Stock||---|
|4514P||40 µl (4 western blots)||---||In Stock||---|
|4514||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||44||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, F=Flow Cytometry
Specificity / Sensitivity
Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb detects endogenous levels of SEK1/MKK4 phosphorylated at Ser257 .
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser257/Thr261 of human SEK1/MKK4.
Western blot analysis of extracts from various cell lines, untreated or treated with sorbitol or UV, as indicated, using Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb.
SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 3346 SEK1 (5C10) Rabbit mAb
- 9151 Phospho-SEK1/MKK4 (Thr261) Antibody
- 9152 SEK1/MKK4 Antibody
- 9155 Phospho-SEK1/MKK4 (Ser80) Antibody
- 9156 Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.