Cell Signaling Technology

Product Pathways - Adhesion

Cool1/βPix Antibody #4515

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Hm Mk Endogenous 81 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Cool1/βPix Antibody detects endogenous levels of total cool1/βPix protein. The antibody does not cross-react with cool2/αPix.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues in the center of human cool1/βPix. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat, NIH/3T3, ZR-75, MCF-7 and COS cells using Cool1/βPix Antibody.

Background

Cool/Pix proteins comprise a family of guanine nucleotide exchange factors (GEFs) localized to focal adhesions. The family consists of two isoforms, cool2/αpix and cool1/βPix, the latter having two splice variants that vary in their carboxy termini (1). Cool1/βPix, like other GEFs, has a DH (Dbl homology) domain, which allows binding of small GTPases and GDP/GTP exchange, and a PH (Pleckstrin homology) domain, which is important in regulating subcellular localization. Cool1/βPix also has an SH3 domain, which binds to the PAK kinase, a downstream effector of cdc42 and Rac (3,4). Phosphorylation of cool1/βPix by PAK2 downstream of MAPK signaling alters the localization of a complex containing PAK2 and cool-1/βPix, regulating formation of growth cones in response to growth factors (4). Growth factor induced activation of Rac1 via cool1/βPix was later shown to occur independently of subcellular localization (5). Endothelin-1 stimulation of mesangial cells stimulates the protein kinase A (PKA) pathway, resulting in translocation of cool-1/βPix and activation of cdc42 (6).

  1. Koh, C.G. et al. (2001) J. Cell Sci. 114, 4239-4251.
  2. Manser, E. et al. (1998) Mol. Cell 1, 183-192.
  3. Bagrodia, S. and Cerione, R.A. (1999) Trends Cell Biol. 9, 350-355.
  4. Shin, E.Y. et al. (2002) J. Biol. Chem. 277, 44417-44430.
  5. Shin, E.Y. et al. (2004) J. Biol. Chem. 279, 1994-2004.
  6. Chahdi, A. et al. (2005) J. Biol. Chem. 280, 578-584.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products