Product Pathways - Apoptosis / Autophagy
Bok Antibody #4521
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H M R Mk | 18 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Bok Antibody detects endogenous levels of total Bok protein. The antibody does not cross-react with endogenous levels of other Bcl-2 family members.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues within a central region of Bok. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HT-1080 (human) and NBT-II (rat) cells using Bok Antibody.
Background
Bok (Bcl-2-related ovarian killer)/ Mtd is a pro-apoptotic Bcl-2 family member identified in a yeast two-hybrid screen using Mcl-1 as the bait (1). Bok contains conserved Bcl-2 homology domains, BH1, BH2, and BH3. In yeast two-hybrid assays, Bok intereacted with some (Mcl-1, BHRF1, and Bfl-1) but not other (Bcl-2, Bcl-xL and Bcl-w) anti-apoptotic family members (1, 2). Likewise, Bok induced apoptosis is blocked by Mcl-1 and BHFR1 but not by Bcl-2 or Bcl-xL (1, 2). Bok was originally desribed to be predominately expressed in reproductive tissues such as ovary, testis and uterus (1). Subsequent studies have found selective expression of Bok in a number of adult and development tissues (2).
- Hsu, S. Y. et al. (1997) Proc. Natl. Acad. Sci. 94, 12401-12406.
- Inohara, N. et al. (1998) J. Biol. Chem. 273, 8705-8710.
Application References
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