Product Pathways - DNA Damage
Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb #4526
|4526L||300 µl (30 western blots)||---||In Stock||---|
|4526S||100 µl (10 western blots)||---||In Stock||---|
|4526||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb detects endogenous levels of ATM only when phosphorylated at Ser1981.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser1981 of human ATM.
Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair (1). Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA double stranded breaks. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair. Known substrates include p53, Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, H2A.X, SMC1, FANCD2, Rad17, Artemis, Nbs1, and the I-2 regulatory subunit of PP1 (1,2). Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage (3,4).
- Xu, X. et al. (2004) . Journal of Biological Chemistry 279, 34091-34094. Applications: Western Blotting.
- Zhu, H. et al. (2010) PLoS One 5, e12055. Applications: Western Blotting.
- Niida, H. et al. (2010) Genes Dev 24, 333-8. Applications: Western Blotting.
- Hawkes, W.C. et al. (2012) J Cell Biochem 113, 61-9. Applications: Western Blotting.
- Unno, J. et al. (2013) Cancer Sci 104, 703-10. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This product is sold under license, U.S. Patent Publication numbers 2003/0077661 and 2003/0157572, from St. Jude Childrens Research Hospital.