Product Pathways - DNA Damage
Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb #4526
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | H | 350 | Mouse | IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb detects endogenous levels of ATM only when phosphorylated at Ser1981.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic phospho-peptide (KLH-coupled) corresponding to residues around Ser1981 of human ATM.
Western Blotting
Western blot analysis of extracts from M059J cells, untreated, treated with doxorubicin, or treated with bleomycin, using Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb.
Background
Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair (1). Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA double stranded breaks. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis and DNA repair. Known substrates include p53, Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, H2AX, SMC1, FANCD2, Rad17, Artemis, Nbs1 and the I-2 regulatory subunit of PP1 (1,2). Mutations in the corresponding ATM gene results in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation and a higher frequency of chromosome breakage (3,4).
- Lee, J.H. and Paull, T.T. (2007) Oncogene 26, 7741-8.
- Tang, X. et al. (2008) Mol Cell Biol Epub ahead of print.
- Shiloh, Y. (1997) Annu Rev Genet 31, 635-62.
- Petrini, J.H. (2000) Curr Opin Cell Biol 12, 293-6.
Application References
- Xu, X. et al. (2004) Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1. Journal of Biological Chemistry 279, 34091-34094. This article references the use of Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb in the following applications: Western Blotting
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This Product is sold under license, U.S.Patent Publication Nos. 2003/0077661and 2003/0157572, from St. JudeChildrens Research Hospital.
