Product Pathways - Cytoskeletal Signaling
Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) #4528
|4528S||100 µl (50 tests)||---||In Stock||---|
|4528||carrier free and custom formulation / quantity||email request|
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|IF-P||1:100||Human, Rat, Monkey||Endogenous||Mouse IgG1|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: IF-P=Immunofluorescence (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
* Product-specific protocol.
Specificity / Sensitivity
Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A431 cells. This antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.
Flow cytometric analysis of Jurkat (blue) and MCF-7 cells (green) using Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate).
Confocal immunofluorescent analysis of MCF-7 cells using Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and Histone H3 Antibody #9715 (green).
Confocal immunofluorescent analysis of paraffin-embedded SK-OV-3 xenograft using Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue). Red= Propidium iodide (PI)/RNase Staining Solution.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The unconjugated antibody #4545 reacts with keratins 4, 5, 6, 8, 10, 13 and 18 from human, rat and monkey. CST expects that Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) will also recognize the same keratins in these species.
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.