Product Pathways - Cytoskeletal Signaling
Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) #4528
| Applications | Reactivity | Source | Isotype |
|---|---|---|---|
| IF-IC IF-P F | H | Mouse | IgG1 |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
IF-P=Immunofluorescence (Paraffin)
F=Flow Cytometry
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.
Source / Purification
Monoclonal antibody (isotype: IgG1) is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from A431 cells. The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-5. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.
Flow Cytometry
Flow cytometric analysis of Jurkat (blue) and MCF-7 cells (green) using Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate). Bottom panel shows Pan-Keratin expression levels in Jurkat and MCF-7 cell lines by Western blot using Pan-Keratin (C11) Mouse mAb #4545. GAPDH (14C10) Rabbit mAb #2118 was used as a loading control.
IF-IC
Confocal immunofluorescent analysis of MCF-7 cells using Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue) and Histone H3 Antibody #9715 (green).
IF-P
Confocal immunofluorescent analysis of paraffin-embedded H3255 xenograft using Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue) and Histone H3 Antibody #9715 (red).
Description
Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody, #4545 reacts with keratins 4, 5, 6, 8, 10, 13 and 18 from human, rat and monkey. CST expects that Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) will also recognize the same keratins in these species.
Background
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratins assemble into filaments, forming heterodimers of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles, which make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613.
- Ramaekers, F.C. and Bosman, F.T. (2004) J. Pathol. 204, 351-354.
- Lane, E.B. and McLean, W.H. (2004) J. Pathol. 204, 355-366.
- Zatloukal, K. et al. (2004) J. Pathol. 204, 367-376.
- Owens, D.W. and Lane, E.B. (2004) J. Pathol. 204, 377-385.
Application References
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Companion Products
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
