Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) #4528

Applications Reactivity Sensitivity Isotype
IF-IC IF-P F H R Mk Endogenous Mouse IgG1

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)  IF-P=Immunofluorescence (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A431 cells. This antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat (blue) and MCF-7 cells (green) using Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and Histone H3 Antibody #9715 (green).

IF-P

IF-P

Confocal immunofluorescent analysis of paraffin-embedded H3255 xenograft using Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and Histone H3 Antibody #9715 (red).


Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The unconjugated antibody #4545 reacts with keratins 4, 5, 6, 8, 10, 13 and 18 from human, rat and monkey. CST expects that Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) will also recognize the same keratins in these species.

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).

  1. Moll, R. et al. (1982) Cell 31, 11-24.
  2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
  3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
  4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
  5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
  6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.

Application References

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Companion Products

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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