Product Pathways - MAPK Signaling
Phospho-TAK1 (Thr184/187) Antibody #4531
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) (C) (B) (X) (Z) | Endogenous | 82 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
C=Chicken
B=Bovine
X=Xenopus
Z=Zebra Fish
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-TAK1 (Thr184/187) Antibody detects endogenous levels of TAK1 only when phosphorylated at both threonine 184 and threonine 187. This antibody weakly cross-reacts with TAK1 singly phosphorylated at threonine 184.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr184 and Thr187 of human TAK1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293 IL-1R cells treated with IL-1alpha (20 ng/ml) or IL-1beta (10 ng/ml) and Calyculin A (50 nM) for 10 minutes, using Phospho-TAK1 (Thr184/187) Antibody.
Background
TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGFbeta, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-kappaB pathway by interacting with TRAF6 and phosphorylating the NF-kappaB inducing kinase (NIK) (2).
TAK1 activation requires multiple phosphorylations in its activation loop. Mutations of Thr187 and Thr184, residues located in the activation loop of TAK1, impairs phosphorylation of both TAK1 and TAB1 and reduces the kinase activity of TAK1, suggesting that autophosphorylation of these residues is necessary for TAK1 activation (4).
- Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
- Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
- Shibuya, H. et al. (1996) Science 272, 1179-1182.
- Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
- Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.
Application References
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Companion Products
- 4536 Phospho-TAK1 (Thr187) Antibody
- 4537 Phospho-TAK1 (Thr184) Antibody
- 4508 Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb
- 4505 TAK1 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
