Product Pathways - Apoptosis
Daxx (25C12) Rabbit mAb #4533
|4533S||100 µl (10 western blots)||---||In Stock||---|
|4533||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||110||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry)
Species predicted to react based on 100% sequence homology: Monkey, Bovine, Dog.
Specificity / Sensitivity
Daxx (25C12) Rabbit mAb detects endogenous levels of total Daxx protein. While Daxx has a calculated MW of 81 kDa, it has been shown to run at an apparent MW of 110 kDa at least in part due to post-translational hyper-phosphorylation (5).
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Gln255 of Daxx.
Western blot analysis of extracts from K562 (human), A20 (mouse) and PC12 (rat) cell lines using Daxx (25C12) Rabbit mAb.
Daxx is a ubiquitously expressed protein that was originally identified through a yeast two-hybrid screen as an interactor with the cytoplasmic domain of Fas. It was found to enhance Fas-mediated apoptosis and activate the JNK pathway (1). However, additional studies have revealed that Daxx is actually a nuclear protein localizing to promyelocytic leukemia oncogenic domains (PODs) (2,3). Nuclear interactions have since been observed with CENP-C (4), Pax3 (5), DNA methyltransferase I (6) and chromatin-associated proteins, including histone deacetylase II, H2A, H2B, H3, H4 and Dek (7). Roles for Daxx have been suggested in transcriptional repression and cell cycle control. Loss of Daxx in mice leads to embryonic lethality with extensive developmental apoptosis, suggesting a role for Daxx directly or indirectly in suppressing cell death (5). Furthermore, inhibition of Daxx expression using RNAi has confirmed Daxx to be anti-apoptotic and to repress transcriptional activity of targets including NF-κB and E2F-1 (8).
- Yang, X. et al. (1997) Cell 89, 1067-1076.
- Torii, S. et al. (1999) EMBO J. 18, 6037-6049.
- Li, H. et al. (2000) Mol. Cell Biol. 20, 1784-1796.
- Pluta, A.F. et al. (1998) J. Cell Sci. 111, 2029-2041.
- Hollenbach, A.D. et al. (1999) EMBO J. 18, 3702-3711.
- Michaelson, J.A. et al. (1999) Gene Dev. 13, 1918-1923.
- Hollenbach, A.D. et al. (2002) J. Cell Sci. 115, 3319-3330.
- Michaelson, J.A. and Leder, P. (2003) J. Cell Sci. 116, 345-352.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.