Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

Daxx (25C12) Rabbit mAb #4533

Applications Reactivity MW (kDa) Source Isotype
W IF-IC H M R (Mk) (B) (Dg) 120 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Daxx (25C12) Rabbit mAb detects endogenous levels of total Daxx protein.

Source / Purification

Rabbit monoclonal antibodies were prepared from spleens of rabbits immunized with a synthetic peptide (KLH-coupled) corresponding to a region surrounding Gln255 of Daxx.

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 (human), A20 (mouse) and PC12 (rat) cell lines using Daxx (25C12) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Daxx (25C12) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Background

Daxx is a ubiquitously expressed protein that was originally identified through a yeast two-hybrid screen as an interactor with the cytoplasmic domain of Fas. It was found to enhance Fas-mediated apoptosis and activate the JNK pathway (1). However, additional studies have revealed that Daxx is actually a nuclear protein localizing to promyelocytic leukemia oncogenic domains (PODs) (2,3). Nuclear interactions have since been observed with CENP-C (4), Pax3 (5), DNA methyltransferase I (6) and chromatin-associated proteins, including histone deacetylase II, H2A, H2B, H3, H4 and Dek (7). Roles for Daxx have been suggested in transcriptional repression and cell cycle control. Loss of Daxx in mice leads to embryonic lethality with extensive developmental apoptosis, suggesting a role for Daxx directly or indirectly in suppressing cell death (5). Furthermore, inhibition of Daxx expression using RNAi has confirmed Daxx to be anti-apoptotic and to repress transcriptional activity of targets including NF-κB and E2F-1 (8).

  1. Yang, X. et al. (1997) Cell 89, 1067-1076.
  2. Torii, S. et al. (1999) EMBO J. 18, 6037-6049.
  3. Li, H. et al. (2000) Mol. Cell Biol. 20, 1784-1796.
  4. Pluta, A.F. et al. (1998) J. Cell Sci. 111, 2029-2041.
  5. Hollenbach, A.D. et al. (1999) EMBO J. 18, 3702-3711.
  6. Michaelson, J.A. et al. (1999) Gene Dev. 13, 1918-1923.
  7. Hollenbach, A.D. et al. (2002) J. Cell Sci. 115, 3319-3330.
  8. Michaelson, J.A. and Leder, P. (2003) J. Cell Sci. 116, 345-352.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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