Product Pathways - Adhesion
MUC1 (VU4H5) Mouse mAb #4538
|W IHC-P IF-IC F||H||Endogenous||230, 400||Mouse IgG1|
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
MUC1 (VU4H5) Mouse mAb detects endogenous levels of total MUC1. This antibody does not cross-react with other mucin proteins.
Source / Purification
Monoclonal antibody (isotype: IgG1k) is produced by immunizing a BALB/c mouse with MUC1 60 mer tandem repeats (BSA conjugated).
Western blot analysis of extracts from PL45 and MCF-7 cells using MUC1 (VU4H5) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using MUC1 (VU4H5) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MUC1 (VU4H5) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using MUC1 (VU4H5) Mouse mAb.
Flow cytometric analysis of MCF-7 cells, using MUC1 (VU4H5) Mouse mAb (blue) compared to a nonspecifc negative control antibody (red).
Confocal immunofluorescent analysis of MCF7 and MDA-MB-231 cells using MUC1 (VU4H5) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation (1). MUC1 (or mucin 1) is aberrently overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a cytoplasmic tail of 72 residues (1). MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation (2). MUC1 also interacts with beta-catenin, which is regulated by GSK-3beta, PKCdelta and Src through phosphorylation at Ser44, Thr41 and Tyr46 of MUC1 cytoplasmic tail (3-5). Overexpression of MUC1 potentiates transformation (6), and attenuates stress induced apoptosis through the Akt or p53 pathways (7,8).
- Baldus, S. E. et al. (2004) Crit. Rev. Clin. Lab. Sci. 41, 189-231.
- Schroeder, J. A. et al. (2001) J. Biol. Chem. 276, 13057-13064.
- Li, . et al. (1998) Mol. Cell. Biol. 18, 7216-7224.
- Li, Y. et al. (2001) J. Biol. Chem. 276, 6061-6064.
- Ren, J. et al. (2002) J. Biol. Chem. 277, 17616-17622.
- Schroeder, J. A. et al. (2004) Oncogene 23, 5739-5747.
- Raina, D. et al. (2004) J. Biol. Chem. 279, 20607-20612.
- Wei, X. et al. (2005) Cancer Cell 7, 167-178.
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For Research Use Only. Not For Use In Diagnostic Procedures.