Cell Signaling Technology

Product Pathways - Adhesion

MUC1 (VU4H5) Mouse mAb #4538

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P IF-IC F H Endogenous 230, 400 Mouse IgG1

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MUC1 (VU4H5) Mouse mAb detects endogenous levels of total MUC1. This antibody does not cross-react with other mucin proteins.

Source / Purification

Monoclonal antibody (isotype: IgG1k) is produced by immunizing a BALB/c mouse with MUC1 60 mer tandem repeats (BSA conjugated).

Western Blotting

Western Blotting

Western blot analysis of extracts from PL45 and MCF-7 cells using MUC1 (VU4H5) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using MUC1 (VU4H5) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MUC1 (VU4H5) Mouse mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using MUC1 (VU4H5) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF-7 cells, using MUC1 (VU4H5) Mouse mAb (blue) compared to a nonspecifc negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 and MDA-MB-231 cells using MUC1 (VU4H5) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation (1). MUC1 (or mucin 1) is aberrently overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a cytoplasmic tail of 72 residues (1). MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation (2). MUC1 also interacts with beta-catenin, which is regulated by GSK-3beta, PKCdelta and Src through phosphorylation at Ser44, Thr41 and Tyr46 of MUC1 cytoplasmic tail (3-5). Overexpression of MUC1 potentiates transformation (6), and attenuates stress induced apoptosis through the Akt or p53 pathways (7,8).

  1. Baldus, S. E. et al. (2004) Crit. Rev. Clin. Lab. Sci. 41, 189-231.
  2. Schroeder, J. A. et al. (2001) J. Biol. Chem. 276, 13057-13064.
  3. Li, . et al. (1998) Mol. Cell. Biol. 18, 7216-7224.
  4. Li, Y. et al. (2001) J. Biol. Chem. 276, 6061-6064.
  5. Ren, J. et al. (2002) J. Biol. Chem. 277, 17616-17622.
  6. Schroeder, J. A. et al. (2004) Oncogene 23, 5739-5747.
  7. Raina, D. et al. (2004) J. Biol. Chem. 279, 20607-20612.
  8. Wei, X. et al. (2005) Cancer Cell 7, 167-178.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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