Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

EOMES Antibody #4540

Applications Reactivity Sensitivity MW (kDa) Source
W M (H) (R) (Mk) Endogenous 70 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

EOMES Antibody detects endogenous levels of total EOMES protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequences at the amino terminus of human EOMES. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from E6.5 and E10.5 stage mouse embryos using EOMES Antibody.

Background

The T-box family of transcription factors is named for their shared homology with the DNA binding domain of the mouse brachyury (T) gene product. Members of this family bind DNA and are capable of transcriptional activation. They also have evolutionarily conserved expression patterns and roles in embryonic development, primarily mesoderm development (1). EOMES, or Tbr2 (T-box brain 2), is a master regulator of mesoderm formation that is also essential for trophoblast formation, gastrulation, neurogenesis and the differentiation of certain T cell subsets. Embryos from EOMES knock-out mice die soon after implantation due to their inability to develop a trophoblast (2,3). Conditional neural knock out mice show defects in development of a specific population of neural progenators known as Intermediate Progenator Cells (IPCs) that give rise only to neurons (4,5). These cells are formed from the radial glia in the ventricular and sub-ventricular zones of the cortex. Expression of EOMES increases as cells develop from radial glia to IPCs and then decreases as IPCs progress to neurons. Recent evidence suggests that EOMES and IPCs may also play a role neurogenesis in the adult hippocampal SGZ (5). EOMES is also a key transcription factor for memory T cells and for full effector differentiation of CD8+ T cells (6). Expression of EOMES is induced in CD8+ T cells following viral infection and bacterial infection where sufficient IL-12 has been produced to elicit acute host cell response (7).

  1. Showell, C. et al. (2004) Dev Dyn 229, 201-18.
  2. Russ, A.P. et al. (2000) Nature 404, 95-9.
  3. Strumpf, D. et al. (2005) Development 132, 2093-102.
  4. Englund, C. et al. (2005) J Neurosci 25, 247-51.
  5. Hodge, R.D. et al. (2008) J Neurosci 28, 3707-17.
  6. Takayanagi, M. et al. (2003) Rheumatol Int 23, 315-8.
  7. Takemoto, N. et al. (2006) J Immunol 177, 7515-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products