Product Pathways - Neuroscience
Phospho-MAP2 (Ser136) Antibody #4541
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-F | H M R Mk | Endogenous | 75, 82, 280 | Rabbit |
Applications Key:
W=Western Blotting
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-MAP2 (Ser136) Antibody detects endogenous levels of all isoforms of MAP2 only when phosphorylated at serine 136. This antibody does not cross-react with phosphorylated tau.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser136 of human MAP2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from PC12 cells, untreated or nocodazole-treated, using Phospho-MAP2 (Ser136) Antibody (upper) or MAP2 Antibody #4542 (lower).
Western Blotting
Western blot analysis of extracts from COS cells, untransfected (lane 1) or MAP2C-transfected (lanes 2,3), using Phospho-MAP2 (Ser136) Antibody (upper) or MAP2 Antibody #4542 (lower). In lane 3, the lysate was treated with lamda phosphatase to demonstrate phospho-specificity of the antibody. (Human MAP2C construct is a generous gift from Dr. B. Shafit-Zagardo, Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY.)
IF-F
Confocal microscopic images of mouse cerebellum stained with Phospho-MAP2 (Ser136) Antibody (red) and CREB (86B10) Mouse mAb #9104 (blue).
Background
Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. Serine 136, threonine 1620 and threonine 1623 of MAP2 are phosphorylated by GSK3 and p44/42 MAP kinases (2,3). Phosphorylation at threonine 1620/1623 by GSK3 inhibits MAP2 association with microtubules and microtubule stability (3).
- Sanchez, C. et al. (2000) Prog. Neurobiol. 61, 133-168.
- Berling, B. et al. (1994) Eur. J. Cell Biol. 64, 120-130.
- Sanchez, C. et al. (2000) Eur. J. Cell Biol. 79, 252-260.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4542 MAP2 Antibody
- 4544 Phospho-MAP2 (Thr1620/1623) Antibody
- 9101 Phospho-p44/42 MAPK (Thr202/Tyr204) Antibody
- 9102 p44/42 MAP Kinase Antibody
- 9106 Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) Mouse mAb
- 9107 p44/42 MAP Kinase (3A7) Mouse mAb
- 9331 Phospho-GSK-3α/β (Ser21/9) Antibody
- 9336 Phospho-GSK-3β (Ser9) Antibody
- 9337 Phospho-GSK-3α (Ser21) (46H12) Mouse mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
