Product Pathways - Neuroscience
Phospho-MAP2 (Ser136) Antibody #4541
PhosphoSitePlus® protein, site, and accession data: MAP2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-F | H R Mk (M) | Endogenous | 75, 82, 280 | Rabbit |
Applications Key:
W=Western Blotting
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Phospho-MAP2 (Ser136) Antibody detects endogenous levels of all isoforms of MAP2 only when phosphorylated at serine 136. This antibody does not cross-react with phosphorylated tau.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser136 of human MAP2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from PC12 cells, untreated or nocodazole-treated, using Phospho-MAP2 (Ser136) Antibody (upper) or MAP2 Antibody #4542 (lower).
Western Blotting
Western blot analysis of extracts from COS cells, untransfected (lane 1) or MAP2C-transfected (lanes 2,3), using Phospho-MAP2 (Ser136) Antibody (upper) or MAP2 Antibody #4542 (lower). In lane 3, the lysate was treated with lamda phosphatase to demonstrate phospho-specificity of the antibody. (Human MAP2C construct is a generous gift from Dr. B. Shafit-Zagardo, Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY.)
IF-F
Confocal immunofluorescent analysis of rat brain, untreated (left) or λ-phosphatase treated (right), using Phospho-MAP2 (Ser136) Antibody (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3).
- Sanchez, C. et al. (2000) Prog. Neurobiol. 61, 133-168.
- Berling, B. et al. (1994) Eur. J. Cell Biol. 64, 120-130.
- Sanchez, C. et al. (2000) Eur. J. Cell Biol. 79, 252-260.
Application References
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Companion Products
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
