Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-MAP2 (Thr1620/1623) Antibody #4544

Applications Reactivity Sensitivity MW (kDa) Source
W H R (M) Transfected Only 82, 280 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-MAP2 (Thr1620/1623) Antibody detects transfected MAP2 (at a dilution of up to 1:20,000) only when phosphorylated at threonines 1620/1623. This antibody does not cross-react with phosphorylated tau.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr1620/1623 of human MAP2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected (lane 1), or MAP2C transfected (lanes 2,3), using Phospho-MAP2 (Thr1620/1623) Antibody (upper) or MAP2 Antibody #4542 (lower). In lane 3, the lysate was treated with lamda phosphatase to demonstrate phospho-specificity of the antibody. (Human MAP2C construct is a generous gift from Dr. B. Shafit-Zagardo, Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY.)

Background

Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. Serine 136, threonine 1620 and threonine 1623 of MAP2 are phosphorylated by GSK3 and p44/42 MAP kinases (2,3). Phosphorylation at threonine 1620/1623 by GSK3 inhibits MAP2 association with microtubules and microtubule stability (3).

  1. Sanchez, C. et al. (2000) Prog. Neurobiol. 61, 133-168.
  2. Berling, B. et al. (1994) Eur. J. Cell Biol. 64, 120-130.
  3. Sanchez, C. et al. (2000) Eur. J. Cell Biol. 79, 252-260.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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