Product Pathways - Cytoskeletal Signaling
Pan-Keratin (C11) Mouse mAb #4545
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC IF-P F | H R Mk | 46, 55 | Mouse | IgG1 |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
IF-P=Immunofluorescence (Paraffin)
F=Flow Cytometry
Reactivity Key:
H=Human
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Pan-Keratin (C11) Mouse mAb detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.
Source / Purification
Monoclonal antibody (isotype: IgG1) is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from A431 cells.
Western Blotting
Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells, using Pan-Keratin (C11) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
IF-P
Confocal immunofluorescent image of paraffin-embedded human colon carcinoma labeled with Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratins assemble into filaments, forming heterodimers of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles, which make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613.
- Ramaekers, F.C. and Bosman, F.T. (2004) J. Pathol. 204, 351-354.
- Lane, E.B. and McLean, W.H. (2004) J. Pathol. 204, 355-366.
- Zatloukal, K. et al. (2004) J. Pathol. 204, 367-376.
- Owens, D.W. and Lane, E.B. (2004) J. Pathol. 204, 377-385.
Application References
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