Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Pan-Keratin (C11) Mouse mAb #4545

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P IF-IC IF-P F H R Mk Endogenous 46-62 Mouse IgG1

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  IF-P=Immunofluorescence (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Pan-Keratin (C11) Mouse mAb detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.

Source / Purification

Monoclonal antibody (isotype: IgG1) is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from A431 cells.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF7 cells, using Pan-Keratin (C11) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

IF-P

IF-P

Confocal immunofluorescent image of paraffin-embedded human colon carcinoma labeled with Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).

  1. Moll, R. et al. (1982) Cell 31, 11-24.
  2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
  3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
  4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
  5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
  6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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