Product Pathways - Cytoskeletal Signaling
Keratin 8/18 (C51) Mouse mAb #4546
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P F | H Mk | Endogenous | 46 Keratin 18. 55 Keratin 8. | Mouse IgG1 |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4546:
- Flow, IHC / Paraffin, Western Blotting
Specificity / Sensitivity
Keratin 8/18 (C51) Mouse mAb detects endogenous levels of total keratins 8 and 18. The antibody does not cross-react with other keratins.
Source / Purification
Monoclonal antibody (isotype: IgG1) is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from HeLa cells.
Western Blotting
Western blot analysis of extracts from various cell lines, using Keratin 8/18 (C51) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Keratin 8/18 (C51) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Keratin 8/18 (C51) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung, using Keratin 8/18 (C51) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Keratin 8/18 (C51) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Keratin 8/18 (C51) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells, using Keratin 8/18 (C51) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).
Background
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
