Product Pathways - Cytoskeletal Signaling
Keratin 18 (DC10) Mouse mAb #4548
PhosphoSitePlus® protein, site, and accession data: K18
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H | Endogenous | 46 | Mouse IgG1 |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Keratin 18 (DC10) Mouse mAb detects endogenous levels of total keratin 18 protein. The antibody does not cross-react with other keratins.
Source / Purification
Monoclonal antibody (isotype: IgG1) is produced by immunizing mice with human PMC-42 breast carcinoma cells.
Western Blotting
Western blot analysis of extracts from HeLa and A431 cells, using Keratin 18 (DC10) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using Keratin 18 (DC10) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Keratin 18 (DC10) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human benign prostate hyperplasia, using Keratin 18 (DC10) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of HeLa cells, using Keratin 18 (DC10) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HepG2 (left) and SH-SY5Y (right) cells using Keratin 18 (DC10) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
