Product Pathways - MAPK Signaling
Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Alexa Fluor® 488 Conjugate) #4551
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| F | H M R | Endogenous | 40 | Mouse IgG1 |
Applications Key:
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-p38MAPK(T180/Y182)(28B10) Mouse mAb (Alexa Fluor® 488 Conjugate) detects p38 MAP kinase only when dually phosphorylated at Thr180 and Tyr182. This antibody does not appreciably cross-react with the corresponding phosphorylated forms of either p44/42 MAPK (Erk1/2) or SAPK/JNK. It does not detect nonphosphorylated p38 MAP kinase.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr180/Tyr182 of human p38 MAP kinase. Antibody was conjugated to Alexa Fluor® 488 under optimum conditions with an F/P ratio of 2-6.
Description
Cell Signaling Technology Antibody conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct Flow Cytometric analysis of human and mouse cells.The unconjugated antibody #9216 reacts with, among others, human, mouse and rat phospho-p38MAPK(T180/Y182). CST expects that Phospho-p38MAPK (T180/Y182) (28B10) Mouse mAb (Alexa Fluor® 488 Conjugate) will also recognize p44/42 MAPK in these species.
Background
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).
- Rouse, J. et al. (1994) Cell 78, 1027-1037.
- Han, J. et al. (1994) Science 265, 808-811.
- Lee, J.C. et al. (1994) Nature 372, 739-746.
- Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
- Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
- Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
- Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
Application References
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Companion Products
- 4552 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Alexa Fluor® 647 Conjugate)
- 9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb
- 9211 Phospho-p38 MAPK (Thr180/Tyr182) Antibody
- 9212 p38 MAP Kinase Antibody
- 9219 Immobilized Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb
- 9820 p38 MAP Kinase Assay Kit (Nonradioactive)
- 9913 Phospho-p38 MAPK Pathway Sampler Kit
- 4555 p38α MAPK (5F11) Mouse mAb (Alexa Fluor® 647 Conjugate)
- 4556 p38α MAPK (5F11) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 9213 p38 MAP Kinase Control Cell Extracts
- 9214 p38δ MAP Kinase Antibody
- 9215 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb
- 9217 p38α MAP Kinase (5F11) Mouse mAb
- 9218 p38α MAP Kinase Antibody
- 9228 p38α MAP Kinase (L53F8) Mouse mAb
- 2307 p38γ MAP Kinase Antibody
- 2308 p38δ MAP Kinase (10A8) Rabbit mAb
- 2371 p38α MAP Kinase (7D6) Rabbit mAb
- 9210 PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit
- 4631 Phospho-p38 MAPK (Thr180/Tyr182)(12F8) Rabbit mAb (IHC Preferred)
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.