Product Pathways - Tyrosine Kinase / Adaptors
Phospho-TNK1 (Tyr277) Antibody #4553
|W||H||Endogenous||72 TNK1, 58 TNK1-C17orf61||Rabbit|
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-TNK1 (Tyr277) Antibody detects endogenous levels of TNK1 protein only when phosphorylated at Tyr277. This antibody may cross-react with tyrosine phosphorylated EGF receptor.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence around Tyr277 of human TNK1. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from L-540 cells using Phospho-TNK1 (Tyr277) Antibody (upper and middle) or TNK1 (C44F9) Rabbit mAb #4570 (lower). The middle membrane was treated with calf intestinal alkaline phosphatase (CIP) before antibody probing. L-540 cells express a 58 kDa TNK1-C17orf61 fusion protein containing 466 amino acids from the amino terminus of TNK1 [Gu, T.L. et al. (2010) Leukemia, in press]. TNK1 kinase was constitutively activated in these cells.
Tyrosine kinase non-receptor 1 (TNK1) is related to the Ack1 (TNK2) non-receptor kinase that binds cdc42 and inhibits GTPase activity of this cell cycle regulator. TNK1 is broadly expressed in embryogenic tissues and leukemia cell lines, but is restricted to select adult tissues (1). TNK1 is a putative 72 kDa protein comprised of an N-terminal kinase domain, a central SH3 domain and a proline-rich tail. Interaction with PLCγ in vitro indicates a possible role in phospholipid signal transduction pathways (2). Though the exact mechanism is currently unclear, active TNK1 may play a role in regulating cell death by preventing TNF-α-induced NF-κB transactivation (3).
Phosphorylation of TNK1 on Tyr277 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (4) and also reported independently in another publication using MS technology (5). Phosphorylation of TNK1 at Tyr277 was observed in select carcinoma cell lines and in tumors. A constitutively active, truncated TNK1 kinase resulting from fusion between the TNK1 and C17orf61 genes is seen in some cells (5). For additional information visit PhosphoSitePlus™, CST's modification site knowledgebase, at www.phosphosite.org.
- Hoehn, G.T. et al. (1996) Oncogene 12, 903-13.
- Felschow, D.M. et al. (2000) Biochem Biophys Res Commun 273, 294-301.
- Azoitei, N. et al. (2007) Oncogene 26, 6536-45.
- Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
- Gu, T.L. et al. (2010) Leukemia 24, 861-5.
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- 4570 TNK1 (C44F9) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.