Product Pathways - Cytoskeletal Signaling
Keratin 19 (BA17) Mouse mAb #4558
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IHC-P F | H | 40 | Mouse | IgG1 |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Keratin 19 (BA17) Mouse mAb detects endogenous levels of total keratin 19 protein. The antibody does not cross-react with other keratin proteins.
Source / Purification
Monoclonal antibody is produced by immunizing mice with detergent-insoluble extract of human mammary epithelial organoids.
Western Blotting
Western blot analysis of extracts from various cell types using Keratin 19 (BA17) Mouse mAb. As expected, the protein is absent in A431 and HeLa cells.
IHC-P (paraffin)
Immunhistochemical analysis of paraffin-embedded human breast carcinoma using Keratin 19 (BA17) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded MCF-7 cells (positive, left) and A431 cells (negative, right), using Keratin 19 (BA17) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of A431 cells (blue) and MCF-7 cells (green) using Keratin 19 (BA17) Mouse mAb.
Background
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratins assemble into filaments, forming heterodimers of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles, which make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613.
- Ramaekers, F.C. and Bosman, F.T. (2004) J. Pathol. 204, 351-354.
- Lane, E.B. and McLean, W.H. (2004) J. Pathol. 204, 355-366.
- Zatloukal, K. et al. (2004) J. Pathol. 204, 367-376.
- Owens, D.W. and Lane, E.B. (2004) J. Pathol. 204, 377-385.
Application References
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