Cell Signaling Technology

Product Pathways - Translational Control

TFII-I Antibody #4562

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P H M Mk Endogenous 135, 138 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TFII-I Antibody detects endogenous levels of total TFII-I (all isoforms).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human TFII-I. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, HEK293, U-937, COS, C2C12 and WEHI-231 cells, using TFII-I Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using TFII-I Antibody.

Background

TFII-I (also known as SPIN and BAP-135) is a mutifunctional transcription factor that facilitates basal transcriptional machinery assembly at the core promotor region, as well as the assembly of the transcriptional activator complex at upstream regulatory sites (1). Four isoforms of TFII-I (alpha, beta, gamma, and delta) form homo- or heteromeric complexes, which may perform different functions on different promoters (1). In B cells, cross-linking of BCR (B cell receptors) leads to TFII-I phosphorylation at Tyr248 by Btk (2). This phosphorylation disrupts the association of Btk and TFII-I and enhances TFII-I transcriptional activity and nuclear localization (2). In nonlymphoid cells, TFII-I is phosphorylated at Tyr248 by Src dependent kinase or JAK2 (3,4). PKG (cGMP-dependent kinase) interacts with and phosphorylates TFII-I at Ser371 and 743, which also promotes TFII-I transcription activity (5). TFII-I activity is also modulated by HDAC3 (Histone Deacetylase 3) through a physical interaction between the two proteins (6).

  1. Roy, A.L. (2001) Gene 274, 1-13.
  2. Novina, C. D. et al. (1999) Mol. Cell. Biol. 19, 5014-5024.
  3. Kim, D. and Cochran, B.H. (2001) Mol. Cell. Biol. 21, 3387-3397.
  4. Cheriyath, V. et al. (2002) J. Biol. Chem. 277, 22798-22805.
  5. Casteel, D. E. et al. (2002) J. Biol. Chem. 277, 32003-32014.
  6. Wen, Y. et al. (2003) J. Biol. Chem. 278, 1841-1847.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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