Product Pathways - Cytoskeletal Signaling
Phospho-MYPT1 (Thr853) Antibody #4563
|4563S||100 µl (10 western blots)||---||In Stock||---|
|4563P||40 µl (4 western blots)||---||In Stock||---|
|4563||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Hamster, Monkey, Dog||Endogenous||140||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Phospho-MYPT1 (Thr853) Antibody detects endogenous levels of MYPT1 only when phosphorylated at Thr853. The antibody cross-reacts with an unidentified protein at 40 kDa.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr853 of human MYPT1. Antibodies are purified using protein A and peptide affinity chromatography.
Western blot analysis of extracts from various cell types using Phospho-MYPT1 (Thr853) Antibody.
Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist (1).
The myosin phosphatase holoenzyme is composed of three subunits: PP1c, a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase), and a 20 kDa subunit of unknown function (M20). MYPT binding to PP1cδ alters the conformation of the catalytic cleft and increases enzyme activity and specificity (2). Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed, while MYPT2 expression appears to be exclusive to heart and brain (3). Related family members include MBS85, MYPT3, and TIMAP (4).
Myosin phosphatase regulates the interaction of actin and myosin in response to signaling through the small GTPase Rho. Rho activity inhibits myosin phosphatase via Rho-associated kinase (ROCK). Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization (5,6).
- Roudier, E. et al. (2009) J Physiol 587, 4105-19. Applications: Western Blotting.
- Hudson, C.A. et al. (2012) Mol Hum Reprod 18, 265-79. Applications: Western Blotting.
- Ramachandran, C. et al. (2011) Invest Ophthalmol Vis Sci 52, 1474-85. Applications: Western Blotting.
- Bernal, P.J. et al. (2011) Am J Physiol Lung Cell Mol Physiol 300, L874-86. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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