Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Dynamin-I (3G4B6) Mouse mAb #4565

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 100 Mouse IgG2a

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Dynamin-I (3G4B6) Mouse mAb detects endogenous levels of total dynamin-I protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant carboxy-terminal fragment of human dynamin-I.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Dynamin-I (3G4B6) Mouse mAb.

Background

Dynamin is a family of large GTPases that has been implicated in the formation of vesicles of both the endocytotic and secretory processes (1). Dynamin plays an important role in the internalization of cell surface receptors, a process that attenuates the response to extracellular signals. It has been illustrated that dynamin interacts with signaling proteins such as Src, PLCγ, PKC and G-proteins. PKC and Src phosphorylate dynamin, and its phosphorylation may regulate the endocytosis of cell surface receptors (2,3).

  1. McNiven, M.A. et al. (2000) Trends Biochem. Sci. 25, 115-120.
  2. Powell, K.A. et al. (2000) J. Biol. Chem. 275, 11610-11617.
  3. Ahn, S. et al. (1999) J. Biol. Chem. 274, 1185-1188.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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