Product Pathways - Tyrosine Kinase/ Adaptors
Axl (C44G1) Rabbit mAb #4566
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP F | H Mk | 138 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
F=Flow Cytometry
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Axl (C44G1) Rabbit mAb detects endogenous levels of total Axl protein. The antibody does not cross-react with related proteins.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with recombinant human Axl-GST fusion protein.
Background
Axl, Sky and Mer are three members of a recently characterized receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets S6K and Akt, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).
- Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
- Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
- Lee, W. P. et al. (2002) Oncogene 21, 329-336.
- Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4939 Axl (C2B12) Rabbit mAb
- 4977 Axl Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide