Product Pathways - Tyrosine Kinase/ Adaptors
Axl (C44G1) Rabbit mAb #4566
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP F | H Mk | 138 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
F=Flow Cytometry
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Axl (C44G1) Rabbit mAb detects endogenous levels of total Axl protein. The antibody does not cross-react with related proteins.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with recombinant human Axl-GST fusion protein.
Western Blotting
Western blot analysis of extracts from human skeletal muscle cells (hSkMC), PAE and HUVEC cells using Axl (C44G1) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of HUVEC cells using Axl (C44G1) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Background
Axl, Sky and Mer are three members of a recently characterized receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets S6K and Akt, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).
- Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
- Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
- Lee, W. P. et al. (2002) Oncogene 21, 329-336.
- Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.
Application References
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