Product Pathways - Tyrosine Kinase / Adaptors
Axl (C44G1) Rabbit mAb #4566
PhosphoSitePlus® protein, site, and accession data: Axl
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP F | H Mk | Endogenous | 138 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
F=Flow Cytometry
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4566:
- Flow, Immunoprecipitation, Western Blotting
Specificity / Sensitivity
Axl (C44G1) Rabbit mAb detects endogenous levels of total Axl protein. The antibody does not cross-react with related proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant human fragment of the Axl protein.
Western Blotting
Western blot analysis of extracts from human skeletal muscle cells (hSkMC), PAE and HUVEC cells using Axl (C44G1) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of HUVEC cells using Axl (C44G1) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Background
Axl, Sky, and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).
- Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
- Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
- Lee, W. P. et al. (2002) Oncogene 21, 329-336.
- Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4939 Axl (C2B12) Rabbit mAb
- 4977 Axl Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
