Cell Signaling Technology

Product Pathways - Metabolism

Fumarase (D9C5) Rabbit mAb #4567

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Hm Mk Endogenous 49 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Fumarase (D9C5) Rabbit mAb recognizes endogenous levels of total fumarase protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly354 of human fumarase protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untransfected (-) or transfected with a Myc-tagged cDNA expression construct encoding human fumarase (hFumarase-Myc; +), using Fumarase (D9C5) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Fumarase (D9C5) Rabbit mAb.

Background

Fumarate hydratase (Fumarase, FH) is canonically regarded as a mitochondrial enzyme of the tricarboxylic acid cycle that catalyzes fumarate to malate (1,2). Studies have demonstrated that deficiencies in FH can lead to hereditary leiomyomatosis and renal cell cancer (1,3) or, in homozygous germline mutations, severe neurological and muscular disorders (1,2). There also exists a cytosolic fumarase isoenzyme that is involved in DNA damage repair (3).

  1. Isaacs, J.S. et al. (2005) Cancer Cell 8, 143-53.
  2. Bourgeron, T. et al. (1994) J Clin Invest 93, 2514-8.
  3. Yogev, O. et al. (2010) PLoS Biol 8, e1000328.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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