Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Cortactin (Tyr421) Antibody #4569

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Hm Mk B Endogenous 80-85 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Cortactin (Tyr421) Antibody detects endogenous levels of cortactin protein only when phosphorylated at Tyr421.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr421 of human cortactin. Antibodies are purified using protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa or NIH/3T3 cells, untreated or treated with hydrogen peroxide, using Phospho-Cortactin (Tyr421) Antibody in the presence of no peptide (A), a phospho-cortactin (Tyr421) peptide (B) or a nonphospho-cortactin peptide (C).

Background

Cortactin is a cortical actin binding protein. Its amino-terminal acidic domain (NTA) associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3 (1,2). Cortactin is involved in signaling events that coordinate actin reorganization during cell movement. The human cortactin homologue EMS1 is overexpressed in numerous cancers with poor patient prognosis (3). Cortactin may also play an important role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network (4).Cortactin is phosphorylated at tyrosine residues 421, 466, and 482. Tyrosine phosphorylation of cortactin regulates cell motility (5), rac1-mediated actin dynamics (6), cadherin-dependent adhesion (7), chemokine trafficking and chemokine-dependent chemotaxis (8).

  1. Du, Y. et al. (1998) Mol Cell Biol 18, 5838-51.
  2. Naisbitt, S. et al. (1999) Neuron 23, 569-82.
  3. Rodrigo, J.P. et al. (2000) Clin Cancer Res 6, 3177-82.
  4. Weed, S.A. and Parsons, J.T. (2001) Oncogene 20, 6418-34.
  5. Huang, C. et al. (1998) J Biol Chem 273, 25770-6.
  6. Head, J.A. et al. (2003) Mol Biol Cell 14, 3216-29.
  7. El Sayegh, T.Y. et al. (2004) J Cell Sci 117, 5117-31.
  8. Luo, C. et al. (2006) J Biol Chem 281, 30081-93.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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