Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

TNK1 (C44F9) Rabbit mAb #4570

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 72 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TNK1 (C44F9) Rabbit mAb detects endogenous levels of TNK1 protein. This antibody does not cross-react with other related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence around Pro97 of human TNK1.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT-116 cells using TNK1 (C44F9) Rabbit mAb.

Background

Tyrosine kinase non-receptor 1 (TNK1) is related to the Ack1 (TNK2) non-receptor kinase that binds cdc42 and inhibits GTPase activity of this cell cycle regulator. TNK1 is broadly expressed in embryogenic tissues and leukemia cell lines, but is restricted to select adult tissues (1). TNK1 is a putative 72 kDa protein comprised of an N-terminal kinase domain, a central SH3 domain and a proline-rich tail. Interaction with PLCγ in vitro indicates a possible role in phospholipid signal transduction pathways (2). Though the exact mechanism is currently unclear, active TNK1 may play a role in regulating cell death by preventing TNF-α-induced NF-κB transactivation (3).

  1. Hoehn, G.T. et al. (1996) Oncogene 12, 903-13.
  2. Felschow, D.M. et al. (2000) Biochem Biophys Res Commun 273, 294-301.
  3. Azoitei, N. et al. (2007) Oncogene 26, 6536-45.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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