Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Lyn (5G2) Mouse mAb #4576

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 56 Mouse IgG2b

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Lyn (5G2) Mouse mAb detects endogenous levels of total Lyn protein. This antibody may cross-react with other Src-family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant His-tagged human Lyn protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Lyn (5G2) Mouse mAb.

Background

Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn in vivo is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8).

  1. Yamanashi, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
  2. Yamanashi, Y. et al. (1991) Science 251, 192-194.
  3. Burkhardt, A.L. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 7410-7414.
  4. Ren, C.L. et al. (1994) J. Exp. Med. 179, 673-680.
  5. Wang, J. et al. (1996) J. Exp. Med. 184, 831-838.
  6. Chan, V.W. et al. (1997) Immunity 7, 69-81.
  7. Hibbs, M. L. et al. (1995) Cell 83, 301-311.
  8. Seo, S.J. et al. (2001) J. Immunol. 166, 3710-3723.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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