Product Pathways - Cell Cycle / Checkpoint
BACH1/BRIP1 Antibody #4578
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H Mk | Endogenous | 145 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
BACH1/BRIP1 Antibody detects endogenous levels of human BACH1 protein.
Source / Purification
BACH1/BRIP1 Antibody is produced by immunizing rabbits with a synthetic peptide corresponding to amino acids near the carboxy terminus of human BACH1. Antibodies are purified by Protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from SH-SY5Y, Saos-2 and Jurkat cells using BACH1/BRIP1 Antibody.
Background
BACH1, also known as BRIP1 and FANCJ, is a DNA helicase involved in repair of DNA cross-links and double strand breaks (1-3). Interaction between phosphorylated BACH1 and BRCA1 is required for DNA damage-induced checkpoint signaling (3,4). Originally identified as a breast cancer susceptibility gene (1), the BACH1 gene is mutated in Fanconi anemia (5), a recessive disorder characterized by multiple congenital abnormalities, progressive bone marrow failure and high cancer risk/predisposition. The interaction with BRCA1 and the existence of mutations in patients with early onset breast cancer indicate that BACH1 may act as a tumor suppressor.
- Cantor, S.B. et al. (2001) Cell 105, 149-60.
- Litman, R. et al. (2005) Cancer Cell 8, 255-65.
- Peng, M. et al. (2006) Oncogene 25, 2245-53.
- Shiozaki, E.N. et al. (2004) Mol Cell 14, 405-12.
- Kennedy, R.D. and D'Andrea, A.D. (2005) Genes Dev 19, 2925-40.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
