Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

BACH1/BRIP1 Antibody #4578

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 145 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BACH1/BRIP1 Antibody detects endogenous levels of human BACH1 protein.

Source / Purification

BACH1/BRIP1 Antibody is produced by immunizing rabbits with a synthetic peptide corresponding to amino acids near the carboxy terminus of human BACH1. Antibodies are purified by Protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y, Saos-2 and Jurkat cells using BACH1/BRIP1 Antibody.

Background

BACH1, also known as BRIP1 and FANCJ, is a DNA helicase involved in repair of DNA cross-links and double strand breaks (1-3). Interaction between phosphorylated BACH1 and BRCA1 is required for DNA damage-induced checkpoint signaling (3,4). Originally identified as a breast cancer susceptibility gene (1), the BACH1 gene is mutated in Fanconi anemia (5), a recessive disorder characterized by multiple congenital abnormalities, progressive bone marrow failure, and high cancer risk/predisposition. Research investigators have concluded that BACH1 interactions with BRCA1 and the presence of BACH1 mutations in patients with early onset breast cancer indicate that BACH1 may act as a tumor suppressor (6).Phosphorylation of BACH1 at Thr1133 is thought to be involved in regulation of the replication checkpoint and is required for the interaction of BACH1 with TopBP1 (7).

  1. Cantor, S.B. et al. (2001) Cell 105, 149-60.
  2. Litman, R. et al. (2005) Cancer Cell 8, 255-65.
  3. Peng, M. et al. (2006) Oncogene 25, 2245-53.
  4. Shiozaki, E.N. et al. (2004) Mol Cell 14, 405-12.
  5. Kennedy, R.D. and D'Andrea, A.D. (2005) Genes Dev 19, 2925-40.
  6. Cantor, S.B. and Guillemette, S. (2011) Future Oncol 7, 253-61.
  7. Gong, Z. et al. (2010) Mol Cell 37, 438-46.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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