Product Pathways - Apoptosis
Survivin (71G4B7) Rabbit mAb (Alexa Fluor® 555 Conjugate) #4580
|4580S||100 µl (50 tests)||---||In Stock||---|
|4580||carrier free and custom formulation / quantity||email request|
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|IF-IC||1:50||Human, Mouse, Rat||Endogenous||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Survivin (71G4B7) Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total survivin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys60 of human survivin protein. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Survivin (71G4B7) Rabbit mAb #2808.
Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6).
- Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200.
- Li, F. et al. (1998) Nature 396, 580-584.
- Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466.
- O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.
- Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.
- Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.