Product Pathways - Apoptosis / Autophagy
Phospho-Bim (Ser69) Antibody #4581
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M (R) (Mk) (Dg) | Endogenous | 26 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Bim (Ser69) Antibody detects endogenous levels of Bim protein only when phosphorylated at Ser69.
Source / Purification
Polyclonal antibodies are prepared by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser69 of human Bim protein (Ser65 in mouse and rat). Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Raji cells, untreated or TPA-treated for 30 min, using Phospho-Bim (Ser69) Antibody (upper), or Bim Antibody #2819 (lower).
Background
Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains (1,2). Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1 and BHRF-1 (1,2). Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated (3-6). Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL and BimS (1). The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BimEL and BimL isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis (7). Apoptotic activity of these longer isoforms may be regulated by phosphorylation (8,9). Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity.
Erk1/2-dependent phosphorylation of BimEL at Ser69 (Ser65 in mouse and rat) in response to growth factor stimulation can promote its proteasome-mediated degradation and enhance cell survival (6,10,11).
- O'Connor, L. et al. (1998) EMBO J 17, 384-95.
- Hsu, S.Y. et al. (1998) Mol Endocrinol 12, 1432-40.
- Bouillet, P. et al. (2002) Nature 415, 922-6.
- Whitfield, J. et al. (2001) Neuron 29, 629-43.
- Dijkers, P.F. et al. (2000) Curr Biol 10, 1201-4.
- Ley, R. et al. (2003) J Biol Chem 278, 18811-6.
- Puthalakath, H. et al. (1999) Mol Cell 3, 287-96.
- Lei, K. and Davis, R.J. (2003) Proc Natl Acad Sci U S A 100, 2432-7.
- Putcha, G.V. et al. (2003) Neuron 38, 899-914.
- Luciano, F. et al. (2003) Oncogene 22, 6785-6793.
- Marani, M. et al. (2004) Oncogene 23, 2431-2441.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2819 Bim Antibody
- 2870 Bcl-2 (50E3) Rabbit mAb
- 2827 Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb
- 9941 Pro-Survival Bcl-2 Family Antibody Sampler Kit (Human Specific)
- 9942 Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
