Product Pathways - Cell Cycle / Checkpoint
Ku70 (D10A7) Rabbit mAb #4588
|4588S||100 µl (10 western blots)||---||In Stock||---|
|4588||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||70||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Hamster, Bovine, Pig.
Specificity / Sensitivity
Ku70 (D10A7) Rabbit mAb recognizes endogenous levels of total Ku70 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val294 of mouse Ku70 protein.
Ku is a heterodimeric protein composed of two subunits (Ku70 and Ku80) originally identified by researchers as autoantigens associated with several autoimmune diseases including scleroderma, polymyositis, and systemic lupus erythematosus (1). Ku is an abundant, ubiquitously expressed nuclear protein that binds to and stabilizes the ends of DNA at telomeres or double-stranded DNA breaks (2-5). The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). DNA-PK has been shown to phosphorylate many proteins, including p53, serum response factor, c-Jun, c-Fos, c-Myc, Oct-1, Sp-1, and RNA polymerase II (1,8). The combined activities of Ku70/Ku80 and DNA-PK implicate Ku in many cellular functions, including cell cycle regulation, DNA replication and repair, telomere maintenance, recombination, and transcriptional activation.
- Tuteja, R. and Tuteja, N. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 1-33.
- Blier, P.R. et al. (1993) J. Biol. Chem. 268, 7594-7601.
- Jin, S. and Weaver, D.T. (1997) EMBO J. 16, 6874-6885.
- Boulton, S.J. and Jackson, S.P. (1998) EMBO J. 17, 1819-1828.
- Gravel, S. et al. (1998) Science 280, 741-744.
- Cao, Q.P. et al. (1994) Biochemistry 33, 8548-8557.
- Lees-Miller, S.P. et al. (1990) Mol. Cell Biol. 10, 6472-6481.
- Collis, S.J. et al. (2005) Oncogene 24, 949-961.
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