Product Pathways - Metabolism
Insulin Antibody #4590
PhosphoSitePlus® protein, site, and accession data: insulin
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| IHC-P IF-F IF-IC F | H M R | Endogenous | 6 | Rabbit |
Applications Key:
IHC-P=Immunohistochemistry (Paraffin)
IF-F=Immunofluorescence (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4590:
- Flow, IHC / Paraffin, Immunofluorescence
Specificity / Sensitivity
Insulin Antibody detects endogenous levels of total insulin protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human insulin. Antibodies are purified by protein A and peptide affinity chromatography.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat pancreas, showing staining of β-cells using Insulin Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse pancreas, showing staining of β cells, using Insulin Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human pancreas, showing staining of β cells, using Insulin Antibody.
Flow Cytometry
Flow cytometric analysis of untreated β-TC cells, using Insulin Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of beta-TC-6 and HeLa cells using Insulin Antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
IF-F
Confocal immunofluorescent analysis of normal mouse pancreas using Insulin Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2).
- Straub, S.G. and Sharp, G.W. (2002) Diabetes Metab. Res. Rev. 18, 451-463.
- Concannon, P. et al. (1998) Nat. Genet. 19, 292-296.
Application References
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Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
