Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

GATA-1 Antibody #4591

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 43 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GATA-1 Antibody detects endogenous levels of total GATA-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro401 of human GATA-1. Antibodies are purified by peptide affinity chromatography

Western Blotting

Western Blotting

Western blot analysis of total lysates from K562 and HEL cells using GATA-1 Antibody.

Background

GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3).

GATA-1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development (4,5). Mutations in GATA-1 have been linked to many human diseases, including acute megakaryoblastic leukemia in Down syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome (6-9).

  1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
  2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
  3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
  4. Pevny, L. et al. (1991) Nature 349, 257-60.
  5. Fujiwara, Y. et al. (1996) Proc Natl Acad Sci USA 93, 12355-8.
  6. Wechsler, J. et al. (2002) Nat Genet 32, 148-52.
  7. Cantor, A.B. (2005) Int J Hematol 81, 378-84.
  8. Mehaffey, M.G. et al. (2001) Blood 98, 2681-8.
  9. Tubman, V.N. et al. (2007) Blood 109, 3297-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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