Product Pathways - Metabolism
C-Peptide Antibody #4593
PhosphoSitePlus® protein, site, and accession data: insulin
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| IHC-P IHC-F IF-F IF-IC | H M R | Endogenous | 4 | Rabbit |
Applications Key:
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-F=Immunofluorescence (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
C-peptide Antibody detects endogenous levels of total C-peptide protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human C-peptide. Antibodies are purified by protein A and peptide affinity chromatography.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human pancreas, showing staining of β-cells, using C-Peptide Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse pancreas, showing staining of β-cells, using C-Peptide Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat pancreas showing staining of β-cells using C-Peptide Antibody.
IHC-F (frozen)
Immunohistochemical analysis of frozen mouse pancreas using C-Peptide Antibody.
IF-IC
Confocal immunofluorescent analysis of β-TC-6 cells (left) and HeLa cells (right) using C-Peptide Antibody (green). Actin filaments have been labeled with DY-555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-F
Confocal immunofluorescent analysis of mouse pancreas using C-Peptide Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Glucose homeostasis is regulated by hormones. Elevation of blood glucose levels during feeding stimulates insulin release from pancreatic β-cells through a glucose sensing pathway (1). Proinsulin, the insulin precursor molecule, is processed prior to its secretion. Insulin is composed of A-peptide and B-peptide which are joined by a disulfide bond. The center one-third of the molecule is cleaved and released as C-peptide, which has a longer half-life than insulin (2).
- Straub, S.G. and Sharp, G.W. (2002) Diabetes Metab. Res. Rev. 18, 451-463.
- Polonsky, K.S. and Rubenstein, A.H. (1984) Diabetes 33, 486-494.
Application References
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Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
