Product Pathways - Glucose Metabolism
C-Peptide Antibody (IHC Specific) #4593
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| IHC-P IHC-F | H M R | Endogenous | 4 | Rabbit |
Applications Key:
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
C-peptide Antibody detects endogenous levels of total C-peptide protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human C-peptide. Antibodies are purified by protein A and peptide affinity chromatography.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human pancreas, showing staining of β-cells, using C-Peptide Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse pancreas, showing staining of β-cells, using C-Peptide Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat pancreas showing staining of β-cells using C-Peptide Antibody.
IHC-F (frozen)
Immunohistochemical analysis of frozen mouse pancreas using C-Peptide Antibody.
Background
Glucose homeostasis is regulated by hormones. Elevation of blood glucose levels during feeding stimulates insulin release from pancreatic β-cells through a glucose sensing pathway (1). Proinsulin, the insulin precursor molecule, is processed prior to its secretion. Insulin is composed of A-peptide and B-peptide which are joined by a disulfide bond. The center one-third of the molecule is cleaved and released as C-peptide, which has a longer half-life than insulin (2).
- Straub, S.G. and Sharp, G.W. (2002) Diabetes Metab. Res. Rev. 18, 451-463.
- Polonsky, K.S. and Rubenstein, A.H. (1984) Diabetes 33, 486-494.
Application References
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Companion Products
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
