Product Pathways - Jak/Stat Pathway
Stat2 Antibody #4594
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IF-IC F | H | 113 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Stat2 Antibody detects endogenous levels of total Stat2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxyl terminus of human Stat2. Antibodies were purified by protein A and peptide affinity chromatography.
Flow Cytometry
Flow cytometric analysis of K562 cells, using Stat2 Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Immunofluorescent staining of HeLa cells, untreated or treated with IFN-alpha, using Stat2 Antibody.
Background
Stat2 (113-kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via assocations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transciption from the ISRE (5).
- Fu, X. et al. (1992) Biochemistry 89, 7840-7843.
- Ihle, J.N. (2001) Curr.Opin.Cell Biol. 13, 211-217.
- Park, C. et al. (2000) Immunity 13, 795-804.
- Improta, T. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4776-4780.
- Horvath, C. et al. (1996) Molecular and Cellular Biology 16, 6957-6964.
Application References
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Companion Products
- 4441 Phospho-Stat2 (Tyr690) Antibody
- 9939 Stat Antibody Sampler Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide