Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat2 Antibody #4594

Applications Reactivity MW (kDa) Source
W IF-IC F H 113 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Stat2 Antibody detects endogenous levels of total Stat2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxyl terminus of human Stat2. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 and SW620 cells, using Stat2 Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K562 cells, using Stat2 Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Immunofluorescent staining of HeLa cells, untreated or treated with IFN-alpha, using Stat2 Antibody.


Background

Stat2 (113-kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via assocations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transciption from the ISRE (5).

  1. Fu, X. et al. (1992) Biochemistry 89, 7840-7843.
  2. Ihle, J.N. (2001) Curr.Opin.Cell Biol. 13, 211-217.
  3. Park, C. et al. (2000) Immunity 13, 795-804.
  4. Improta, T. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4776-4780.
  5. Horvath, C. et al. (1996) Molecular and Cellular Biology 16, 6957-6964.

Application References

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