Cell Signaling Technology

Product Pathways - NF-kB Signaling

TRIF Antibody #4596

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 98 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TRIF Antibody detects endogenous levels of total human TRIF protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser219 of human TRIF/TICAM-1. Antibodies were purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of HeLa cells, mock transfected or transfected with human TRIF, and from Raji, MOLT-4 and SR cell lines using TRIF Antibody.

Background

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.

TRIF (also termed TICAM-1) is a TIR-domain adaptor protein described to activate NF-κB, IRF3 and trigger IFN-β production (4,5). Studies using dominant negative forms of TRIF and siRNA targeting TRIF show that TRIF functions downstream of both TLR3 and TLR4 in response to dsRNA and LPS respectively (4-6). TRIF recruits TRAF6-TAK1-TAB2 to the receptor complex which leads to NF-κB activation (7). In addition, TRIF can trigger signaling of that lead to the induction of apoptosis (8).

  1. Akira, S. (2003) J Biol Chem 278, 38105-8.
  2. Beutler, B. (2004) Nature 430, 257-63.
  3. Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
  4. Medzhitov, R. et al. (1997) Nature 388, 394-7.
  5. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
  6. Takeuchi, O. et al. (1999) Immunity 11, 443-51.
  7. Alexopoulou, L. et al. (2001) Nature 413, 732-8.
  8. Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
  9. Horng, T. et al. (2001) Nat Immunol 2, 835-41.
  10. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
  11. Muzio, M. et al. (1997) Science 278, 1612-5.
  12. Wesche, H. et al. (1997) Immunity 7, 837-47.
  13. Suzuki, N. et al. (2002) Nature 416, 750-6.
  14. Irie, T. et al. (2000) FEBS Lett 467, 160-4.
  15. Yamamoto, M. et al. (2002) J Immunol 169, 6668-72.
  16. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
  17. Fitzgerald, K.A. et al. (2003) J Exp Med 198, 1043-55.
  18. Jiang, Z. et al. (2004) Proc Natl Acad Sci USA 101, 3533-8.
  19. Kaiser, W.J. and Offermann, M.K. (2005) J Immunol 174, 4942-52.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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