Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat2 Antibody (Mouse Specific) #4597

Applications Reactivity Sensitivity MW (kDa) Source
W M Endogenous 130 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Stat2 Antibody (mouse specific) detects endogenous levels of total mouse Stat2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy terminus of mouse Stat2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from BaF3 cells and mouse thymus, using Stat2 Antibody (mouse specific) #4597.

Background

Stat2 (113-kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via assocations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transciption from the ISRE (5).

  1. Fu, X. et al. (1992) Biochemistry 89, 7840-7843.
  2. Ihle, J.N. (2001) Curr.Opin.Cell Biol. 13, 211-217.
  3. Park, C. et al. (2000) Immunity 13, 795-804.
  4. Improta, T. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4776-4780.
  5. Horvath, C. et al. (1996) Molecular and Cellular Biology 16, 6957-6964.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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