Cell Signaling Technology

Product Pathways - Adhesion

Integrin α4 Antibody #4600

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 70, 140, 150, (180) Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Integrin α4 Antibody detects endogenous levels of integrin α4 mature protein (150kDa), α4 precursor protein (140kDa), as well as 70 kDa cleaved C-terminal α4 fragment.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1027 of human integrin α4. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from MOLT-4 and Jurkat cells using Integrin α4 Antibody.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).A pair of important α4 integrins, α4β1 and α4β7, interact with VCAM-1, fibronectin and MAdCAM-1 at cell adhesions (3). Gene knockout and antibody blocking research reveal that α4 integrins play important roles in embryonic liver and heart development and in fetal lymphocyte homing (4-6). Phosphorylation at Ser988 within the cytoplasmic tail of integrin α4 blocks integrin α4 binding to paxillin and promotes leading edge migration (7,8). Integrin α4 is potentially a key therapeutic target in the treatment of inflammatory bowel disease and multiple sclerosis (9,10).On SDS PAGE, Integrin α4 can migrate at several different apparent molecular sizes, a 150 kDa mature protein, and a 140 kDa precursor protein (a 180 kDa protein also exists under mild non-reducing conditions) (11). α4 has a cleavage site at R558, which results in a small portion of the protein as either a 80 kDa N-terminal or a 70 kDa C-terminal fragment (12).

  1. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
  2. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
  3. Plow, E.F. et al. (2000) J Biol Chem 275, 21785-8.
  4. Bonder, C.S. et al. (2005) Immunity 23, 153-63.
  5. Arroyo, A.G. et al. (1999) Immunity 11, 555-66.
  6. Yang, J.T. et al. (1995) Development 121, 549-60.
  7. Nishiya, N. et al. (2005) Nat Cell Biol 7, 343-52.
  8. Alon, R. et al. (2005) J Cell Biol 171, 1073-84.
  9. Lanzarotto, F. et al. (2006) Drugs 66, 1179-89.
  10. Bartt, R.E. (2006) Curr Opin Neurol 19, 341-9.
  11. Teixidó, J. et al. (1992) J Biol Chem 267, 1786-91.
  12. Pujades, C. et al. (1996) Biochem J 313 ( Pt 3), 899-908.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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