Product Pathways - Neuroscience
Doublecortin Antibody #4604
PhosphoSitePlus® protein, site, and accession data: doublecortin
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-F F | H M R Mk Dm | Endogenous | 45 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-F=Immunofluorescence (Frozen)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dm=D. melanogaster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Doublecortin Antibody detects endogenous levels of total doublecortin protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human doublecortin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from mouse and rat brain, using Doublecortin Antibody.
Flow Cytometry
Flow cytometric analysis of SK-N-MC cells, using Doublecortin antibody (blue) compared to a nonspecific negative control antibody (red).
IF-F
Confocal immunofluorescent analysis of Doublecortin Antibody (red) and GFAP (GA5) Mouse mAb #3670 (green) staining the subventricular zone of the striatum in reperfused ischemic rat brain. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).
- Gleeson, J.G. et al. (1998) Cell 92, 63-72.
- Reiner, O. et al. (2004) Cell Cycle 3, 747-751.
- Gdalyahu, A. et al. (2004) EMBO J. 23, 823-832.
- Schaar, B.T. et al. (2004) Neuron 41, 203-213.
- Tanaka, T. et al. (2004) Neuron 41, 215-227.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
