Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Doublecortin (Ser297) Antibody #4605

Applications Reactivity MW (kDa) Source
W H R (M) 45 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Doublecortin (Ser297) Antibody detects endogenous levels of doublecortin only when phosphorylated at Ser297.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser297 of doublecortin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from fetal rat and fetal human brain using Phospho-Doublecortin (Ser297) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after Western transfer.

Background

Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein, stabilizing and bundling microtubules. A conserved doublecortin domain mediates the interaction with microtubules and interestingly, most missense mutations cluster in this domain (2). JNK, CDK5 and PKA all phosphorylate doublecortin: JNK phosphorylates Thr321, Thr331 and Ser334; PKA phosphorylates Ser47; and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).

  1. Gleeson, J.G. et al. (1998) Cell 92, 63-72.
  2. Reiner, O. et al. (2004) Cell Cycle 3, 747-751.
  3. Gdalyahu, A. et al. (2004) EMBO J. 23, 823-832.
  4. Schaar, B.T. et al. (2004) Neuron 41, 203-213.
  5. Tanaka, T. et al. (2004) Neuron 41, 215-227.

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