Product Pathways - Wnt / Hedgehog / Notch
MAML1 Antibody #4608
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H | Endogenous | 130 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
MAML1 Antibody detects endogenous levels of total MAML1 protein. It does not cross-react with MAML2 and MAML3.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding His801 of human MAML1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of total cell lysates from HeLa and 293 cells, using MAML1 antibody.
IF-IC
Confocal immunofluorescent analysis of MCF-7 cells labeled with MAML1 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Background
Mastermind-like (MAML) family of proteins are homologs of Drosophila Mastermind. The family is composed of three members in mammals: MAML1, MAML2 and MAML3 (1,2). MAML complexes with the intracellular portion of Notch (ICN) and the transcription factor CSL (RBP-Jκ) to regulate Notch target gene expression (3-5). MAML1 also interacts with MEF2C (myocyte enhancer factor 2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthin's tumors (7).
- Wu, L. et al. (2002) Mol. Cell Biol. 22, 7688-7700.
- Lin, S.E. et al. (2002) J. Biol. Chem. 277, 50612-50620.
- Kitagawa, M. et al. (2001) Mol. Cell Biol. 21, 4337-4346.
- Nam, Y. et al. (2003) J. Biol. Chem. 278, 21232-21239.
- Nam, Y. et al. (2006) Cell 124, 973-983.
- Shen, H. et al. (2006) Genes Dev. 20, 675-688.
- Tonon, G. et al. (2003) Nat. Genet. 33, 208-213.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2421 Cleaved Notch1 (Val1744) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
