Cell Signaling Technology

Product Pathways - Neuroscience

Trk (pan) (C17F1) Rabbit mAb #4609

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H M R Endogenous 140 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Trk (pan) (C17F1) Rabbit mAb detects endogenous levels of total Trk protein. This antibody detects TrkA, TrkB and TrkC.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Tyr785 of human TrkA.

Western Blotting

Western Blotting

Western blot analysis of extracts from fetal human brain and mouse brain using Trk (pan) (C17F1) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 cells, untransfected (blue) or TrkA transfected (green), using Trk (pan) (C17F1) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells, wild-type (upper left), NIH/3T3/TrkA (upper right), NIH/3T3/TrkB (lower left) or NIH/3T3/TrkC (lower right) using Trk (pan) (C17F1) Rabbit mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


Background

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).

  1. Pierotti, M.A. and Greco, A. (2006) Cancer Lett 232, 90-8.
  2. Segal, R.A. and Greenberg, M.E. (1996) Annu Rev Neurosci 19, 463-89.
  3. Stephens, R.M. et al. (1994) Neuron 12, 691-705.
  4. Obermeier, A. et al. (1993) EMBO J 12, 933-41.
  5. Obermeier, A. et al. (1994) EMBO J 13, 1585-90.
  6. Yao, R. and Cooper, G.M. (1995) Science 267, 2003-6.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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