Cell Signaling Technology

Product Pathways - Neuroscience

Dysbindin Antibody #4611

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 50 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Dysbindin Antibody detects endogenous levels of total dysbindin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human dysbindin. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of HeLa cells, untransfected or transfected with a construct overexpressing dysbindin, and other cell lines using Dysbindin Antibody.

Background

Dysbindin, or dystrobrevin-binding protein 1, is a coiled-coil-containing protein expressed in muscle and brain that was identified as a binding partner of dystrobrevin (1). Dysbindin upregulates expression of the pre-synaptic proteins SNAP25 and synapsin I, thereby increasing glutamate release and promoting neuronal viability through Akt signaling. In particular, Akt phosphorylation is suppressed with downregulation of dysbindin and increased with upregulation of dysbindin (2). A nonsense mutation of dysbindin causes Hermansky-Pudlak disease, an autosomal recessive disorder characterized by lysosomal storage defects and prolonged bleeding. (2). Genetic variation in the gene encoding dysbindin is strongly associated with schizophrenia and protein levels are reduced in the prefrontal cortex, midbrain and hippocampus of brains from patients with schizophrenia (3,4).

  1. Benson, M.A. et al. (2001) J Biol Chem 276, 24232-41.
  2. Numakawa, T. et al. (2004) Hum Mol Genet 13, 2699-708.
  3. Straub, R.E. et al. (2002) Am J Hum Genet 71, 337-48.
  4. Talbot, K. et al. (2004) J Clin Invest 113, 1353-63.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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