Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

CTMP Antibody #4612

Applications Reactivity Sensitivity MW (kDa) Source
W IP F H M R Endogenous 22, 26 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CTMP Antibody detects endogenous levels of total CTMP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human CTMP. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using the CTMP Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, using CTMP antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Carboxyl-terminal modulator protein (CTMP) is a negative regulator of Akt and was identified as an Akt binding protein. Akt, also referred to as PKB or Rac, plays a critical role in controlling the balance between survival and apoptosis (1-3). This protein kinase, activated by insulin and various growth and survival factors, functions in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4), and by phosphorylation within the carboxy-terminus at Ser473. CTMP binds to the regulatory domain of Akt at the carboxy terminus of the protein and inhibits phosphorylation on Thr308 and Ser473 (5).

  1. Franke , T. F. et al. (1997) Cell 88, 435-437.
  2. Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
  3. Franke, . F. et al. (1995) Cell 81, 727-736.
  4. Alessi, D. R. et al. (1996) EMBO J. 15, 6541-6551.
  5. Maira, S. M. et al. (2001) Science 294, 374-380.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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