Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

MAML2 Antibody #4618

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 160 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

MAML2 Antibody detects endogenous levels of total MAML2 protein. It does not cross-react with MAML1 and MAML3.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Asp800 of human MAML2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from 293, NIH/3T3 and A10 cells, using MAML2 antibody.

Background

Mastermind-like (MAML) family of proteins are homologs of Drosophila Mastermind. The family is composed of three members in mammals: MAML1, MAML2 and MAML3 (1,2). MAML complexes with the intracellular portion of Notch (ICN) and the transcription factor CSL (RBP-Jκ) to regulate Notch target gene expression (3-5). MAML1 also interacts with MEF2C (myocyte enhancer factor 2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthin's tumors (7).

  1. Wu, L. et al. (2002) Mol. Cell Biol. 22, 7688-7700.
  2. Lin, S.E. et al. (2002) J. Biol. Chem. 277, 50612-50620.
  3. Kitagawa, M. et al. (2001) Mol. Cell Biol. 21, 4337-4346.
  4. Nam, Y. et al. (2003) J. Biol. Chem. 278, 21232-21239.
  5. Nam, Y. et al. (2006) Cell 124, 973-983.
  6. Shen, H. et al. (2006) Genes Dev. 20, 675-688.
  7. Tonon, G. et al. (2003) Nat. Genet. 33, 208-213.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

Products