Product Pathways - Wnt / Hedgehog / Notch
MAML2 Antibody #4618
PhosphoSitePlus® protein, site, and accession data: MAML2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 160 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
MAML2 Antibody detects endogenous levels of total MAML2 protein. It does not cross-react with MAML1 and MAML3.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp800 of human MAML2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of total cell lysates from 293, NIH/3T3 and A10 cells, using MAML2 antibody.
Background
Mastermind-like (MAML) family of proteins are homologs of Drosophila Mastermind. The family is composed of three members in mammals: MAML1, MAML2, and MAML3 (1,2). MAML complexes with the intracellular domain of Notch (ICN) and the transcription factor CSL (RBP-Jκ) to regulate Notch target gene expression (3-5). MAML1 also interacts with MEF2C (myocyte enhancer factor 2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthin's tumors (7).
- Wu, L. et al. (2002) Mol. Cell Biol. 22, 7688-7700.
- Lin, S.E. et al. (2002) J. Biol. Chem. 277, 50612-50620.
- Kitagawa, M. et al. (2001) Mol. Cell Biol. 21, 4337-4346.
- Nam, Y. et al. (2003) J. Biol. Chem. 278, 21232-21239.
- Nam, Y. et al. (2006) Cell 124, 973-983.
- Shen, H. et al. (2006) Genes Dev. 20, 675-688.
- Tonon, G. et al. (2003) Nat. Genet. 33, 208-213.
Application References
- Köchert, K. et al. (2011) Oncogene 30, 1831-40. Applications: Western Blotting
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Companion Products
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4147 Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
